The Neuroimmunology team is seeking a motivated and technically-skilled individual with strong CNS cell biology skills to join our Cambridge-based team.
Candidates will collaborate with colleagues across diverse functional areas including genetics, chemical biology, immunology, and pharmacology.
Primary responsibilities include culturing stable cell lines, primary, and iPS-derived microglia to support the study of disease-associated functions and phenotypes that will aid in the identification and validation of Client therapeutic targets to treat neurodegenerative diseases.
Responsibilities:
Assist in developing and performing disease-relevant cell-based functional assays.
Perform high content imaging, biochemical, and molecular profiling techniques.
Communicate data clearly and concisely to teams and stakeholders.
Thrive in a team environment.
Qualifications – Education & Experience:
BS/BA in Biology, Neuroscience, Molecular Biology, Bioengineering, Biotechnology, or equivalent degree.
Minimum of + years of industry or academic experience.
Required Skills & Competencies:
Strong general knowledge of cell culture methods and sterile technique.
Experience working with immortalized cell lines, primary rodent neurons, and glia (, microglia).
Experience with human iPS-derived CNS cell models including microglia and neurons.
Understanding of standard cell transfection techniques using lipid-based and electroporation methodology.
Proficiency in cell staining methods including immunofluorescence and cell structural staining methods.
Experience with method optimization, troubleshooting, and strong critical thinking skills.
Proven experience in image acquisition and microscopy.
Strong working knowledge of mouse neuroanatomy.
Ability to work independently on data analysis, including practical knowledge of statistics.
Preferred Skills:
Experience with plate-based high content imaging microscopy.
Live cell imaging experience.
Demonstrated experience with quantitative image analysis.
Working knowledge of biochemical and molecular biology techniques including protein and RNA extraction, quantitation, and detection (western blot, ELISA, qPCR).
Experience with cellular signaling and functional studies (, phagocytosis, cell motility).
Strong oral, written, and presentation skills; ability to explain concepts clearly in lab and small team settings.